Use of FISH testing to demonstrate nuclear her-2 gene copy numbers

FISH testing for her-2 - negative pattern with approximately equal numbers of red dots marking individual her-2 genes and green dots marking the centromere of Chromosome 17. There is a degree of polysomy in this case with approximately 5 or 6 Chromosome 17s per cell. Original magnification x100 - cropped approx 50%.

'Mouse over' - positive pattern with true gene ampification - large numbers of her-2 gene copies indicated by clusters of red dots and very few green dots.

FISH testing for Her-2 gene copies - 'mouse over' for positive result pattern

FISH testing for her-2 - polysomy (negative)

FISH testing for Her-2 gene copies - polysomy

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